Labbox Agarose D1, low EEO (electro-endosmosis) GEN, 500 g
The Labbox Agarose D1, low EEO (electro-endosmosis) GEN, 500 g is a reliable solution for precise analyses in molecular biology laboratories. When it comes to cleanly resolving DNA, RNA or protein fragments, this product delivers particularly clear band sharpness, minimises interfering distortions and impresses with consistent quality. Anyone who requires efficiency and clear sample analysis in the research or diagnostics field will benefit from its stable gel behaviour and flexible usability in a wide range of applications.
Performance characteristics and areas of application
This agarose is characterised above all by its low electroendosmosis (EEO). In electrophoresis in particular, it is important that the molecules to be analysed migrate evenly and without distortion. The lower EEO value reduces unclean band images, which is crucial for reproducibility in diagnostic and research laboratories. In this way, nucleic acids or certain proteins can be separated efficiently and identified beyond doubt.
Thanks to an optimised particle size, evenly distributed pores are created that can separate both DNA and RNA molecules in different size ranges. The consistently high purity ensures that foreign substances or variations within the agarose particles have hardly any influence on the run results. As a result, users benefit from a high ramp-up speed for routine protocols and save time, especially when processing numerous samples on a daily basis.
The low EEO value also reduces heat generation during the run, resulting in longer stable gels. This is particularly important when increased tension or long running distances are required, for example to separate particularly long DNA fragments. Thanks to its universal applicability in common buffers such as TAE and TBE, the agarose can be easily integrated into existing laboratory workflows.
The most important technical data at a glance:
- Content: 500 g
- Degree of purity: Suitable for demanding molecular biology applications
- CAS: 9012-36-6
- EC: 232-658-1
- Brand: LABKEM
- Low EEO: For minimised band distortion
- TARIC code: 13023100
In addition to these basic values, other parameters such as moisture level or viscosity remain within a range that ensures reproducibility in recurring analyses. A clear focus of this product is to offer users uniform results with little optimisation effort. Such consistent properties are essential when screening large sample quantities, for routine PCR controls in medical laboratories or for research projects in biotechnology.
Certifications and standards
Since various laboratories often have to meet strict quality specifications, this agarose is produced using standardised processes and traceable raw materials. The indication of CAS (9012-36-6) and EC (232-658-1) supports simple traceability. Even without explicitly mentioned certification according to DIN or CE standards, it must be ensured that the product complies with industry standards. Anyone carrying out genetic engineering or diagnostics applications can therefore rely on stable quality that can be used in standard control processes without much additional effort.
Examples of applications
- DNA fragments for PCR controls and restriction analyses: samples can be checked quickly, especially in clinical routine, by separating them on this gel for quality control purposes
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- RNA analyses for research projects: Reliable RNA separation is achieved with suitable buffers and, if necessary, a denaturing step, for example to analyse gene expression patterns.
- Protein fractions: Proteins can also be separated in certain buffer systems if specific fractions are to be isolated for further analysis.
- Clinical diagnostics: The low EEO reduces artefacts and thus creates a clear demarcation of the bands, which is an essential criterion for the evaluation of medical samples.
Agarose is already routinely used in many biotech and research environments to prepare DNA sequencing or verify the results of cloning experiments, among other things. Using various dyes such as ethidium bromide or SYBR? Green (depending on the laboratory standard), bands can be visualised and subsequently documented.
FAQs
How can the powder be dissolved safely
?
Heat a pre-measured amount of agarose in TAE or TBE buffer until everything is dissolved. A microwave or laboratory hotplate are commonly used for this purpose.
What concentration is ideal for common DNA gels?
Mostly 0.8 % to 2 % are used. Lower concentrations facilitate the separation of larger DNA fragments, while higher concentrations clearly separate smaller fragments.
What equipment is required to produce an agarose gel?
A simple heating source and a gel chamber with a comb are usually sufficient for creating sample pockets. A separate device is not absolutely necessary.
Is this agarose also suitable for RNA determination?
Yes, with suitable buffer systems, for example with the addition of denaturing reagents, you can efficiently separate RNA and avoid artefacts caused by secondary structures.
How should the gel be disposed of after the run?
After the electrophoresis run, the gel can be disposed of as solid waste in most laboratories. However, if certain dyes are used, the respective disposal guidelines must be observed.
Conclusion
Thanks to its reduced EEO value and high-quality purity, this agarose offers ideal conditions for the precise separation of nucleic acids and selected proteins. Users in research and diagnostics benefit from a consistently reliable quality that produces clear and reproducible bands. Thanks to its universal use in various buffer systems, switching between different applications is easy; an optimised pore structure ensures that pieces of DNA, RNA or protein migrate consistently and predictably. This solution therefore proves to be time and cost-saving, particularly in laboratory-intensive areas, as it delivers reliable results for both short analyses and extended run times.
