Labbox Agarose E GEN, 500 g

Labbox Agarose E GEN, 500 g

This agarose granulate has been specially developed to achieve highly precise and reproducible results in molecular biology and biotechnology. It is ideal for analysing and separating nucleic acids and proteins and delivers consistent results thanks to its high purity and low endosmosis. Compared to conventional alternatives, this reagent also impresses with its particularly stable gel matrix, which ensures excellent band sharpness and is therefore predestined for reliable diagnostic and research applications.

Reliable results for molecular biology

The product enables differentiated separation of DNA and RNA fragments in different size ranges. Thanks to its high purity, background staining is significantly reduced when using staining methods such as ethidium bromide, SYBR Green or Coomassie. This allows bands to be clearly recognised, which facilitates precise further processing, such as the cutting out of DNA or protein fragments and their subsequent cloning. In addition, the stable gel structure is resistant to mechanical stress and therefore ensures reliable sample handling.

The agarose granules dissolve easily in common buffer solutions and are characterised by quick handling. As soon as the solution has melted, the gel can be poured and used for electrophoretic separation within a short time. A particularly uniform solidification process and the absence of unnecessary impurities make it an ideal choice for academic and industrial research laboratories with high quality standards.

Technical details at a glance

- CAS: 9012-36-6
- EC: 232-658-1
- Purity: High purity, for demanding molecular biology
- High gel strength at low concentration
- TARIC code: 13023100
- Suitable for standard and low melting procedures
- Brand: LABKEM

These key facts make it clear that the product is suitable for both classic applications and innovative methods such as low-melting gels. This means that the range of techniques that can be used is very broad. Thanks to the consistent quality, no extensive adjustments to existing SOPs are necessary, which simplifies the work steps in the laboratory.

Integration into already established workflows is straightforward: Users benefit from the rapid solubility as well as compatibility with different gel concentrations (typically 0.8-2%). This allows laboratories to selectively set the resolution required for their samples. A major advantage is that a clear band structure prevails even with more concentrated gels, which is particularly important when fragment sizes are closer together.

Certifications and standards

Although typical certifications, such as CE markings or DIN standards, are more tailored to mechanical laboratory equipment, agarose production is subject to strict purity and quality assurance. This product is subject to comprehensive quality tests, which include melting point and gel strength. Precise monitoring of these parameters ensures that the material always has the same properties regardless of batch changes and that both research laboratories and industrial users can rely on consistent results in the long term.

Concrete application examples

- Routine analysis of PCR products in genetic research
- Analysis of restriction fragments in plasmid or vector analysis
- Visualisation of RNA bands in diagnostics
- Separation of proteins using agarose gels containing SDS

In addition, the product supports numerous staining methods to display specific proteins or DNA fragments. Thanks to the high purity and low background staining, the gels produced are also suitable for demanding detection steps, for example when using SYBR Green or Coomassie.

FAQs

How should the powder be stored

?

Airtight and dry, ideally at room temperature. This keeps the agarose in optimum condition over a long period of time if used regularly.

Which concentration achieves the best results

Depending on the size of the fragments to be separated, gel concentrations typically vary between 0.8% and 2%. Scientists adjust the concentration to their specific requirements.

Is any special pre-treatment necessary?

No. It is usually sufficient to add the weighed amount to the buffer provided and then heat until the powder is completely dissolved.

Can different colourings be used?

Yes, the agarose can be used with common staining reagents such as ethidium bromide, SYBR Green or Coomassie without any problems. Additional adjustments are generally not required.

How long does the solidification take?

After casting, the gel only needs a few minutes to harden. The electrophoresis setup can then be created and sample analysis can begin immediately.

Conclusion

This agarose granulate impresses with its high purity, reliable gel strength and uncomplicated handling in all routine biotechnological and research applications. With optimum band sharpness and a stable gel structure, it offers precise separation results for DNA, RNA and proteins. Thanks to its fast solubility, low background staining and compatibility with common staining and analysis systems, it is ideal for laboratories that want to organise their workflow efficiently. This reagent is a consistent and high-quality choice, especially in demanding projects where distinctive band separation and repeatability are essential.

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