Labbox Agarose LM-SIEVE GEN, 100 g

Labbox Agarose LM-SIEVE GEN, 100 g

This agarose is specially designed to support high-resolution DNA separations in sensitive molecular biology processes. Due to its particularly low melting temperature and consistent gel structure, it is a reliable option for demanding laboratory applications. Anyone looking for a precise yet flexible agarose in research or diagnostics laboratories will particularly appreciate the clear band visualisations and uncomplicated handling.

High resolution and reliable separation quality

With an optimised composition, this agarose offers exceptional separation efficiency for DNA fragments. The reduction of interfering background signals promotes clean band analysis, which speeds up workflows and increases the reliability of results. Thanks to low inherent fluorescence, samples can be optimally recognised even at low concentrations. This gel matrix is particularly advantageous in procedures that require precise fine differentiation. Critical work steps such as PCR analyses, cloning or mutation screening also benefit from an agarose that carefully separates DNA fragments and does not contain any interfering additives.

Minimal denaturation of sensitive samples is also extremely important. The lower melting temperature means that DNA structures are barely affected, meaning that important sequences are preserved. This has a positive effect on further processing, such as subsequent sequencing. Another advantage is the high compatibility with common dye systems, such as ethidium bromide or SYBR Green. This means that the laboratory remains flexible and can easily integrate different staining methods.

Favourable handling in the laboratory

The stable gel matrix ensures a uniform pore size. This leads to clear separation of even the smallest DNA sections. At the same time, the high separation accuracy reduces the risk of smearing, which enables test series with lower error rates. The simple mixing and pouring of the gel characterises this product, as viscosity and gel time can be easily controlled. This often eliminates the need to perform multiple tests just to find the right settings.

The laboratory routine is simplified by compatibility with the common TAE and TBE buffer systems. This allows existing protocols to remain unchanged. While conventional agaroses require higher temperatures for melting and can therefore have a negative impact on sensitive DNA, this variant ensures gentle treatment of the sample material. This results in considerable time and cost savings, especially in working environments with complex or expensive genetic samples.Certifications and standards

Manufacturing takes place under strictly controlled conditions, often in accordance with ISO 9001 quality management. This ensures that the product remains consistent in terms of purity and structure. Users benefit from validated processes and hold a medium in their hands that is designed for high-quality applications in the biotechnological and molecular biology sector. In addition to certification, the product's labelling (EC: 232-658-1, CAS: 9012-36-6, TARIC 13023100) provides clear identification and simplifies international trade and documentation.

Examples of application

- Separation of short DNA fragments for mutation detection
- Separation of PCR products prior to exact sequencing
- Cloning preparation for plasmids in biotechnology projects
- Identification of specific markers in breeding studies
- Precise quality control during sample preparation in diagnostic laboratories

The flexibility of the gel is not only advantageous for classic DNA analyses. The reliable gel structure also contributes to clean results in demanding applications, such as the analysis of gene libraries or complex proteomics analyses.

Technical data

- Free from DNases and RNases
- Minimal background for clear analyses
- Low melting temperature for sensitive samples
- Optimised pore size for high-resolution separations
- Tough gel matrix for stable handling
- Compatible with ethidium bromide and SYBR Green
- Clear labelling via EC and CAS numbers

Apart from these core data, the agarose remains stable over a wide temperature range, allowing users to adjust the gel concentration as required without sacrificing precision. The pore size significantly influences the migration speed of different DNA fragments, which is why the concentration can be individually adjusted depending on the desired resolution.

FAQs

What is the best way to store the product?

Store it in a dry place at room temperature, ideally protected from light and in well-sealed containers.

Is any buffer suitable?

Yes, TAE and TBE are tried and tested systems. Other common buffers can also be used without any problems and without compromising the separation quality.

Do special disposal methods need to be used?

As a rule, agarose is not considered a hazardous substance. However, always follow internal laboratory safety guidelines and dispose of used gel material in accordance with regulations.

Can the gel strength be customised?

Yes, varying the agarose concentration changes the pore size, which is ideal for DNA fragments of different sizes.

Which staining methods are suitable?

The gel matrix is compatible with many dye systems. Classic variants such as ethidium bromide are just as suitable as modern alternatives.

Conclusion

With Labbox Agarose LM-SIEVE GEN, 100 g, research institutes and diagnostic laboratories benefit from an agarose that delivers precise results and minimises deviations. The low melting temperature protects heat-sensitive DNA, while the high purity reduces interfering influences during analysis. The flexible application options and easy adaptation to different test conditions not only increase the efficiency of the processes, but also improve reliability in every step. This provides the laboratory with a medium that has been developed for a wide range of applications and always enables reproducible results.

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