Labbox three-sugar iron agar (TSI) BAC ISO-6579, ISO-19250, 500 g
This three-sugar iron agar has been specially developed for the reliable detection of enteric bacteria and is ideal for the precise analysis of Salmonella and Shigella thanks to its clear colour changes. It offers high reliability and is ideally suited for microbiological detection in food and water in accordance with ISO-6579 and ISO-19250. The particular advantage over conventional culture media lies in its combined indicator function: laboratories can obtain a comprehensive picture of possible pathogens and their metabolic properties in just a few steps.
Precise analyses thanks to intelligent formulation
The integrated sugar components (glucose, lactose and sucrose) enable different fermentation behaviours to be recorded. At the same time, H?S production can be detected quickly by incorporating iron ions into the medium. This allows black colonies to be displayed, which facilitates the detection of some Salmonella species. This simplified procedure is appreciated in many laboratories as it combines safety and speed.
Technical key features at a glance:
- Contains glucose, lactose and sucrose for differentiated fermentation evaluation
- Supplemented with iron ions for the detection of hydrogen sulphide (H?S)
- High ISO conformity (ISO-6579, ISO-19250) for food and water analysis
- Clear colour changes for reliable identification of pathogens
- Reproducible batch quality for reliable method control
- Long shelf life for economical laboratory operation
- Easy to use thanks to intuitive handling protocols
This mixture of different sugars and iron compounds offers a versatile approach to bacterial differentiation: the formation of yellow, red or black areas in the agar gives a clear indication of whether a bacterial species is acidogenic or forms H?S. This makes it possible, for example, to differentiate between harmless Enterobacteriaceae and problematic isolates such as Salmonella or Shigella.
High reproducibility is particularly important in quality assurance in food processing companies. The triple sugar simplifies evaluation because different parameters (e.g. glucose and lactose utilisation) can be observed separately. This allows targeted detection of potentially dangerous germs, which is of great importance for health protection. This agar preparation also scores highly in research facilities, as it provides important insights into the behaviour of bacterial strains in characterisation, mutation analysis and resistance testing studies. Clinical diagnostics laboratories benefit from the clear differentiation between pathogenic and harmless bacteria, which can be particularly relevant in therapy planning.Norms and quality standards
Compliance with relevant standards contributes significantly to the reliability of the analyses. This product fulfils both ISO-6579 for Salmonella detection and ISO-19250 for microbiological water analyses. It can be used to analyse foodstuffs as well as drinking water, well water or surface water. Users can be sure that they are always working with a legally compliant and standard-compliant medium that has been produced under careful conditions. Each batch undergoes quality control to ensure consistently high standards. This provides laboratories with sound and reliable results, which are crucial for regulatory approvals or internal quality assurance, for example.
Concrete fields of application
- Food inspection: The reliable detection of Salmonella or Shigella strains in meat, eggs or other processed products is made much easier.
- Water testing: Enteric pathogens can be quickly and clearly detected in accordance with ISO-19250, particularly in drinking and bathing water.
- Research and teaching: Universities and research institutes utilise the wide range of differentiation options to investigate the physiology and pathogenicity of individual germs.
- Pharmacy and clinics: If gastrointestinal infections are suspected, the medium offers simple identification of possible pathogens.
Frequently asked questions
How to prepare the powder correctly
Suspend the medium in deionised or distilled water according to the specified mixing ratio. Heat while stirring until the powder is completely dissolved. Then sterilise and allow to harden in sterile Petri dishes.
What are the optimum storage conditions?
The powder is best stored in a cool, dry place away from light. A constant room temperature without extreme fluctuations helps to maintain stability. Ready-poured slabs should be used quickly so that the nutritional quality does not deteriorate.
Is pH adjustment necessary
The product is adjusted so that a constant pH range is achieved. If measurements show that the pH value deviates significantly, you can make a careful sterile adjustment. Further sterile filtration or reheating must be observed in order to avoid contamination.
How can typical germs be distinguished?
Pathogens with strong H?S formation stain the agar surface black, whereas acid-producing bacteria cause a colour change to yellow. Shigella often remains red in certain areas. This makes it easy to recognise which reactions are taking place in the respective culture preparation.
What accessories are required?
In addition to an autoclave for sterilisation and suitable Petri dishes, a pH meter is recommended for occasional checks. Sterile inoculation loops also facilitate the targeted spreading of samples. An efficient incubator provides the necessary conditions for all bacteria to grow.
Conclusion
The three-sugar iron agar offers a high degree of safety and reliability in microbiological analysis thanks to its clever combination of sugar components and iron compounds. This makes it possible to quickly differentiate between various Enterobacteriaceae without the need for time-consuming additional tests. This medium is an ideal choice for testing food and water in particular, but also for research purposes. Its ISO-6579 and ISO-19250 conformity as well as the concise colour changes make evaluation easy and provide safety in everyday laboratory work. Anyone who needs a fast and precise method will find a proven and popular solution that can be seamlessly integrated into professional analysis workflows.
