Laboratory Chromatography Equipment
Manufacturer for Chromatography Equipment
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Laboratory Chromatography - uses
Chromatography (color chromatography) is a chemical process in the laboratory in which a mixture of substances is separated by the different way in which its individual components are distributed in a stationary and mobile phase. Depending on the chromatographic separation method, the stationary phase consists of a solid or a liquid and the mobile phase of a gas or a liquid. The purpose of chromatography is the detection of individual substances in a mixture or the determination of their concentration and the preventive isolation of substances from a mixture of substances for further processing.
Chromatography is used in practice in chemistry, biology, medicine, pharmacy, environmental analysis, criminal forensics, the food or chemical industry. Application examples: In the field of production, chromatography is used, for example, to isolate or purify substances. In chemical analysis it is used to separate mixtures into components that are as homogeneous as possible for the purpose of identifying or quantitatively analyzing samples. Our range of chromatography equipment includes chromatography devices, systems and accessories for the most diverse applications in laboratories, inorganic and organic chemistry, biochemistry, microbiology, biotechnology, food chemistry or environmental chemistry.
Laboratory Chromatography - basic principle and explanation of the process
Chromatography explained simply: Although there are different chemical chromatography processes, the underlying principle can be illustrated relatively simply. A rapidly flowing river (water = mobile phase) usually carries material along with it (= various substances in chromatography). The speed at which this material moves depends on several factors: firstly on the type of material, i.e. small grains of sand are transported faster than larger stones, secondly on the nature of the river bed (= stationary phase), rough surfaces increase the friction on the material carried along and thus reduce the transport speed and finally on the flow speed. So, when a mixture of small grains of sand and large pieces of gravel is shaken into the river, in principle all the fine-grained sand reaches a certain point first, followed by larger particles at certain intervals.
In chromatography, various substances in a mixture become distributed differently. In the mobile phase, a liquid or a gas moves more or less quickly past a solid or liquid stationary phase, separating the contained substances. The separation of the contained substances depends on the affinity to the stationary or mobile phase.
Laboratory Chromatography - types of separation
According to the separation principle, the various types of chromatography can be classified as adsorption, distribution, ion exchanges, affinity, gel permeation or chiral chromatography. For the user in the laboratory and for the selection of instruments, systems and accessories for individual chromatography requirements, a classification according to the mobile phase and the carrier material or physical state of the stationary phase is more helpful.
Paper chromatography: in accordance with its separation method, paper chromatography is a type of liquid chromatography in which the solid, stationary phase is paper. Usually, a paper strip is used that stands vertically in a glass container. The mobile phase as eluent is a liquid which is moved by capillary forces and transports the substances of the substance mixture. Paper chromatography is used in the analysis of substance mixtures.
Thin-layer chromatography: Thin-layer chromatography is also part of liquid chromatography and works methodically according to the same separation principle as paper chromatography. The difference of the separation method is the stationary phase, which in thin-layer chromatography usually consists of aluminum oxide, cellulose or silica gel and is applied very finely to a carrier material (aluminium foil, plastic film, glass plate) on which the substance to be investigated is then separated. The advantages of this type of chromatography are the fast running time and also the high detection sensitivity.
Column chromatography: in laboratory practice column chromatography is considered as liquid chromatography. Its stationary phase usually consists of finely powdered silica gel or aluminium oxide, which is placed in a glass tube and filled with a solvent (often cyclohexane/ethyl acetate) as mobile phase. In principle, in this method, the separated components leave the tube consecutively. Column chromatography is mainly used in the purification of preparations.
High-performance liquid chromatography: high-performance liquid chromatography (HPLC) belongs to the column chromatography group. In analysis, it is the most frequently applied method of separation under high pressure.
Gas chromatography: various processes are used in gas chromatography. Both gas-solid chromatography or the more commonly used gas-liquid chromatography are employed in the laboratory. The carrier material, e.g. silicone oil, is filled into a thin spiral-shaped tube and the gas mixture to be analyzed flows together with the gaseous separation gas (often nitrogen, helium or argon) through the spiral and at the end a detector measures the occurring temperature fluctuations. From these, the components of the substances can be determined. Gas chromatography is used for the detection of gaseous or non-decomposed vaporing substances.
Lab Chromatography - accessories
Our chromatography equipment range includes chromatography devices by reputable laboratory technology manufacturers that are also reliable, safe and convenient to use. The chromatography systems also allow you to replace parts or add accessories. Depending on the model and method, you can order various chromatography components and accessories, such as different carrier materials, blotting/chromatography paper, chromatography columns, glass plates, UV light, capillary pipettes, spray heads, chromatography refrigerators, and chromatography chambers and many other components.
Chromatography (color chromatography) is a chemical process in the laboratory in which a mixture of substances is separated by the different way in which its individual components are distributed in a stationary and mobile phase. Depending on the chromatographic separation method, the stationary phase consists of a solid or a liquid and the mobile phase of a gas or a liquid. The purpose of chromatography is the detection of individual substances in a mixture or the determination of their concentration and the preventive isolation of substances from a mixture of substances for further processing.
Chromatography is used in practice in chemistry, biology, medicine, pharmacy, environmental analysis, criminal forensics, the food or chemical industry. Application examples: In the field of production, chromatography is used, for example, to isolate or purify substances. In chemical analysis it is used to separate mixtures into components that are as homogeneous as possible for the purpose of identifying or quantitatively analyzing samples. Our range of chromatography equipment includes chromatography devices, systems and accessories for the most diverse applications in laboratories, inorganic and organic chemistry, biochemistry, microbiology, biotechnology, food chemistry or environmental chemistry.
Laboratory Chromatography - basic principle and explanation of the process
Chromatography explained simply: Although there are different chemical chromatography processes, the underlying principle can be illustrated relatively simply. A rapidly flowing river (water = mobile phase) usually carries material along with it (= various substances in chromatography). The speed at which this material moves depends on several factors: firstly on the type of material, i.e. small grains of sand are transported faster than larger stones, secondly on the nature of the river bed (= stationary phase), rough surfaces increase the friction on the material carried along and thus reduce the transport speed and finally on the flow speed. So, when a mixture of small grains of sand and large pieces of gravel is shaken into the river, in principle all the fine-grained sand reaches a certain point first, followed by larger particles at certain intervals.
In chromatography, various substances in a mixture become distributed differently. In the mobile phase, a liquid or a gas moves more or less quickly past a solid or liquid stationary phase, separating the contained substances. The separation of the contained substances depends on the affinity to the stationary or mobile phase.
Laboratory Chromatography - types of separation
According to the separation principle, the various types of chromatography can be classified as adsorption, distribution, ion exchanges, affinity, gel permeation or chiral chromatography. For the user in the laboratory and for the selection of instruments, systems and accessories for individual chromatography requirements, a classification according to the mobile phase and the carrier material or physical state of the stationary phase is more helpful.
Paper chromatography: in accordance with its separation method, paper chromatography is a type of liquid chromatography in which the solid, stationary phase is paper. Usually, a paper strip is used that stands vertically in a glass container. The mobile phase as eluent is a liquid which is moved by capillary forces and transports the substances of the substance mixture. Paper chromatography is used in the analysis of substance mixtures.
Thin-layer chromatography: Thin-layer chromatography is also part of liquid chromatography and works methodically according to the same separation principle as paper chromatography. The difference of the separation method is the stationary phase, which in thin-layer chromatography usually consists of aluminum oxide, cellulose or silica gel and is applied very finely to a carrier material (aluminium foil, plastic film, glass plate) on which the substance to be investigated is then separated. The advantages of this type of chromatography are the fast running time and also the high detection sensitivity.
Column chromatography: in laboratory practice column chromatography is considered as liquid chromatography. Its stationary phase usually consists of finely powdered silica gel or aluminium oxide, which is placed in a glass tube and filled with a solvent (often cyclohexane/ethyl acetate) as mobile phase. In principle, in this method, the separated components leave the tube consecutively. Column chromatography is mainly used in the purification of preparations.
High-performance liquid chromatography: high-performance liquid chromatography (HPLC) belongs to the column chromatography group. In analysis, it is the most frequently applied method of separation under high pressure.
Gas chromatography: various processes are used in gas chromatography. Both gas-solid chromatography or the more commonly used gas-liquid chromatography are employed in the laboratory. The carrier material, e.g. silicone oil, is filled into a thin spiral-shaped tube and the gas mixture to be analyzed flows together with the gaseous separation gas (often nitrogen, helium or argon) through the spiral and at the end a detector measures the occurring temperature fluctuations. From these, the components of the substances can be determined. Gas chromatography is used for the detection of gaseous or non-decomposed vaporing substances.
Lab Chromatography - accessories
Our chromatography equipment range includes chromatography devices by reputable laboratory technology manufacturers that are also reliable, safe and convenient to use. The chromatography systems also allow you to replace parts or add accessories. Depending on the model and method, you can order various chromatography components and accessories, such as different carrier materials, blotting/chromatography paper, chromatography columns, glass plates, UV light, capillary pipettes, spray heads, chromatography refrigerators, and chromatography chambers and many other components.