Laboratory Electrophoresis Units
Manufacturer for Electrophoresis Units
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Electrophoresis unit - area of application
Electrophoresis systems are devices that are mainly used in molecular biology, biochemistry, biotechnology, food analysis, veterinary and human medical laboratories. Among the most important applications of the electrophoresis units are the production of polyacrylamide gels, the electrophoretic separation of proteins, nucleic acids, protein staining, DNA/RNA analysis in the form of fragments and DNA sequencing in the laboratory.
Electrophoresis is a laboratory technique in which substance mixtures or particles are separated in an electrical field on the basis of size, weight and electrical charge. For example, in laboratory medicine serum electrophoresis is used for fractionating serum proteins. To diagnose various diseases, doctors can use protein electrophoresis to obtain information about the presence of the relative amounts of protein, albumin, alpha-2, alpha-2, beta-globulin and gamma-globulin in the blood. The electrophoresis apparatuses offered by Elettrofor and BIOTEC FISCHER are primarily suitable for various gel applications and laboratory experiments.
Gel electrophoresis units - functional principle
Gel electrophoresis systems are devices used in the laboratory for gel electrophoresis, a technique that uses a gel as a carrier medium. Agarose or polyacrylamide forms a close-meshed network that slows down the molecules to be separated during their migration in the electric field.
Principle of gel electrophoresis: Electrophoresis takes place in the gel electrophoresis unit by electrophoretic separation through the pores present in the gel, which act like a sieve and whose size determines the migration speed of the molecules present in the gel. Depending on the size and charge of the molecules, they move at different speeds through the gel, which acts as a molecular sieve. The size of the gel pores is determined by the concentration of the basic gel, agarose or polyacrylamide.
In gel electrophoresis units, small, negatively charged molecules (anions) and short DNA fragments migrate fastest towards the positively charged anode. Positively-charged molecules (cations) migrate in the direction of the negatively-charged cathode.
As a reference, a marker of DNA molecules of known length is also present. Natively DNA/RNA fragments are invisible and they are therefore stained either with ethidium bromide or with mega fluorine. Proteins are additionally charged as zwitterions via a detergent such as sodium dodecyl sulfate (SDS), so that a separation according to molecular mass can take place.
Agarose gel: Agarose gels have relatively large pores. The pore size can be regulated by way of the agarose concentration. 1% gels have a pore size of around 150 nanometers, 0.15% gels of around 500 nm. Agarose gel electrophoresis is suitable for the separation of DNA and larger protein molecules. The main area of application is the separation of nucleic acids.
Polyacrylamide gel: polyacrylamide gels are produced through the polymerisation of acrylamide. They have much smaller pores of between 3 and 6 nanometres. The pore size depends on the degree of cross-linking. Polyacrylamide electrophoresis is frequently used in the separation of smaller protein molecules.
Gel electrophoresis systems - types
Gel electrophoresis apparatuses are available in various designs, sizes and models, and different gel electrophoresis systems are used depending on the type of electrophoresis, the field of application, the method, the carrier material and other factors.
Classic gel electrophoresis is carried out as zone electrophoresis. Discontinuous electrophoresis achieves a higher resolution. There are also electrophoresis types such as free-flow electrophoresis, density gradient electrophoresis of affinity electrophoresis.
We classify our gel electrophoresis units according to the gel chamber: agarose gel and polyacrylamide electrophoresis: horizontal gel electrophoresis systems and vertical gel electrophoresis systems.
Electrophoresis apparatus - accessories
Our gel electrophoresis apparatuses are characterised by their robust construction, long service life and reliability - as well as by the possibility of replacing or adding individual parts. Depending on the electrophoresis system, you can order different components and electrophoresis accessories, such as different glass plates, electrophoresis chambers, combs, gel carriers, gel casting systems, gel pouring ramps, spacer sets, contrast plates, and other accessories for the gel electrophoresis systems offered.
Electrophoresis systems are devices that are mainly used in molecular biology, biochemistry, biotechnology, food analysis, veterinary and human medical laboratories. Among the most important applications of the electrophoresis units are the production of polyacrylamide gels, the electrophoretic separation of proteins, nucleic acids, protein staining, DNA/RNA analysis in the form of fragments and DNA sequencing in the laboratory.
Electrophoresis is a laboratory technique in which substance mixtures or particles are separated in an electrical field on the basis of size, weight and electrical charge. For example, in laboratory medicine serum electrophoresis is used for fractionating serum proteins. To diagnose various diseases, doctors can use protein electrophoresis to obtain information about the presence of the relative amounts of protein, albumin, alpha-2, alpha-2, beta-globulin and gamma-globulin in the blood. The electrophoresis apparatuses offered by Elettrofor and BIOTEC FISCHER are primarily suitable for various gel applications and laboratory experiments.
Gel electrophoresis units - functional principle
Gel electrophoresis systems are devices used in the laboratory for gel electrophoresis, a technique that uses a gel as a carrier medium. Agarose or polyacrylamide forms a close-meshed network that slows down the molecules to be separated during their migration in the electric field.
Principle of gel electrophoresis: Electrophoresis takes place in the gel electrophoresis unit by electrophoretic separation through the pores present in the gel, which act like a sieve and whose size determines the migration speed of the molecules present in the gel. Depending on the size and charge of the molecules, they move at different speeds through the gel, which acts as a molecular sieve. The size of the gel pores is determined by the concentration of the basic gel, agarose or polyacrylamide.
In gel electrophoresis units, small, negatively charged molecules (anions) and short DNA fragments migrate fastest towards the positively charged anode. Positively-charged molecules (cations) migrate in the direction of the negatively-charged cathode.
As a reference, a marker of DNA molecules of known length is also present. Natively DNA/RNA fragments are invisible and they are therefore stained either with ethidium bromide or with mega fluorine. Proteins are additionally charged as zwitterions via a detergent such as sodium dodecyl sulfate (SDS), so that a separation according to molecular mass can take place.
Agarose gel: Agarose gels have relatively large pores. The pore size can be regulated by way of the agarose concentration. 1% gels have a pore size of around 150 nanometers, 0.15% gels of around 500 nm. Agarose gel electrophoresis is suitable for the separation of DNA and larger protein molecules. The main area of application is the separation of nucleic acids.
Polyacrylamide gel: polyacrylamide gels are produced through the polymerisation of acrylamide. They have much smaller pores of between 3 and 6 nanometres. The pore size depends on the degree of cross-linking. Polyacrylamide electrophoresis is frequently used in the separation of smaller protein molecules.
Gel electrophoresis systems - types
Gel electrophoresis apparatuses are available in various designs, sizes and models, and different gel electrophoresis systems are used depending on the type of electrophoresis, the field of application, the method, the carrier material and other factors.
Classic gel electrophoresis is carried out as zone electrophoresis. Discontinuous electrophoresis achieves a higher resolution. There are also electrophoresis types such as free-flow electrophoresis, density gradient electrophoresis of affinity electrophoresis.
We classify our gel electrophoresis units according to the gel chamber: agarose gel and polyacrylamide electrophoresis: horizontal gel electrophoresis systems and vertical gel electrophoresis systems.
Electrophoresis apparatus - accessories
Our gel electrophoresis apparatuses are characterised by their robust construction, long service life and reliability - as well as by the possibility of replacing or adding individual parts. Depending on the electrophoresis system, you can order different components and electrophoresis accessories, such as different glass plates, electrophoresis chambers, combs, gel carriers, gel casting systems, gel pouring ramps, spacer sets, contrast plates, and other accessories for the gel electrophoresis systems offered.